ANTIGENIC PROFILES OF PROTEINPOLYSACCHARIDE COMPLEX FROM HUMAN COSTAL CARTILAGE

Proteinpolysaccharide of Bovine Cartilage

Evidence is presented which establishes that chondroitin sulfate and keratan sulfate are part of the same macromolecular proteinpolysaccharide in bovine nasal septum. Degradation of the proteinpolysaccharide with papain releases single chains of chondroitin sulfate with a characteristic residual peptide still attached. A larger molecular weight fragment contains the keratan sulfate which retain...

Proteinpolysaccharides from human articular and costal cartilage.

The proteinpolysaccharides of human costal cartilage.

Water-soluble proteinpolysaccharides, called PPL, can be extracted from bovine nucleus pulposus in yields of 45%, and from bovine nasal cartilage in yields of 37% of the dry tissue weight. From human costal cartilage only 7% can be extracted. The method used to separate PPL from each of the first two tissues into four distinct fractions separates the PPL of human costal cartilage into four frac...

Proteinpolysaccharide complex from bovine nasal cartilage. A comparison of low and high shear extraction procedures.

Two procedures for isolating 80 to 85% of the total hexuronic acid from bovine nasal cartilage as proteinpolvsaccharide complex are described and compared. The first method, termed disruptive, extracts the complex by exhaustive high speed homogenization of the tissue in salt solutions of low ionic strength; proteinpolysaccharides used in most previous investigations were extracted similarly. Th...

Proteinpolysaccharide complex from bovine nasal cartilage. The function of glycoprotein in the formation of aggregates.

Proteinpolysaccharide complex from bovine nasal cartilage exhibits a bimodal distribution of sedimentation coefficients when analyzed in the ultracentrifuge in associative solvents such as 0.5 M guanidinium chloride. The faster sedimenting mode disaggregates in dissociative solvents such as 4 M guanidinium chloride, giving a unimodal centrifugal pattern. Proteinpolysaccharide complex is separat...